VitroFlow

Organ-on-a-Chip made easy with VitroFlow.

The VitroFlow device contains all systems for creating a physiologically relevant environment with oxygen and nutrient supply aswell as temperature control and pump. All parameters are monitored and controlled by sensors. The external chip chamber allows the microscope tobe used freely during the experiment. The chamber itself has interchangeable inlets to allow the connection of a wide variety of chips from different manufacturers.
  • Cost-effective in vitro simulation of human organ function
  • Live microscopy during the experiment
  • Single & Multi-Organ Chips
  • Cloud-based app for automated live imaging of cells and more
  • Free choice of cells and chips
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Kits and accessories to boost your VitroFlow.
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Why choose
VitroFlow?

VitroFlow creates a physiological microenvironment for human cell culture to study cellular interaction, morphological changes and responses toexternal stimuli under physiologically relevant biomechanical conditions for advanced drug screening and disease modelling.

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Free choice of chip and cells

Temperature Control 25–90 °C

24/7-Live-Monitoring

Customizable gas incubation at the microfludic scale

4 seperatly adjustable and analysable flows 2–1000 μL/min

All in one OoC solution — all systems integrated for creating a physiologically relevant microenvironment

Full reproducibility — all parameters monitored and controlled by sensors.

24/7 live monitoring — use of any microscope (phase contrast & fluorescence) possible

Chamber system

Fully Compatible and Customizable Chamber System

The chamber is fully mobile and compatible with standard microscope stages, enabling high-resolution imaging and screening. A microfluidic chip is clamped between two inlay parts. The inlays are fully customizable, allowing precise adaptation to a wide range of chip types—from commercially available formats to proprietary designs.

  • ANSI/SLAS standard plate 
  • Dimensions for microscopy and screening
  • Integrated heating for chip temperature control
  • Customizable insert for different chip formats
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The upper inlay securely holds the chip in place and can be customized to match different chip geometries.

The lower inlay provides stable support for the chip and ensures precise alignment during imaging and screening.

The base serves as the foundation of the chamber, housing integrated functions such as heating and compatibility with standard microscope stages.

VitroFlow.Connect

Maximum control. Minimal Presence.

Our app connects directly to VitroFlow.Bio & allows you to monitor and control your experiments – automated, remote & smart.

VitroFlow.Connect supports the analysis and documentation of microfluidic cell culture experiments – intuitively, reliably and efficiently. Live imaging is automated and data storage is simplified.

  • Less manual intervention - lower error rate
  • Better collaboration across institutes
  • Basis for future AI evaluation tools
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The app continuously records, displays, and saves live sensor data (flow rate, temperature & gas concentration)

App automatically captures and saves images throughout the experiment.

Live & automatic

Camera transmission in real time, storage of cell images at adjustable intervals

All data, always at hand

Sensor values (temperature, flow rate, etc.) are displayed & saved.

Work & analyse remotely

Remote control of experiment parameters & access to saved protocols – ideal for weekends & cross-location teams.

Procedure of an experiment
with VitrofluidiX

1

Define Your Setup

First, you select or create the protocol: define which medium to use, set flow parameters, and assign organ-specific presets. Ready-made templates for common organ models are available, or you can customize your own. Then, simply fill the designated media compartments in the system.

2

Insert the Organ-on-a-Chip

Now it’s time to place your chip. Seed the required organ-specific cells onto the chip of your choice — single-organ, dual-organ, or a custom model. Once prepared, insert the chip into the incubation chamber and connect it to the system.

3

Start the Experiment

With all components in place, you’re ready to launch. Start the protocol and let VitroFlow take over — the organ simulation begins, fully automated and precisely controlled.

4

Analyze in Real Time

VitrofluidiX enables live imaging in fluorescence and phase contrast using microscopes already in your lab. The chip chamber follows the standard well plate format and can stay under the microscope throughout the experiment. Imaging runs continuously and automatically—ensuring real-time insights with no manual steps.

Kits

Kits and accessories to power
your VitroFlow system

Our range of modular kits and accessories is designed to keep your VitroFlow system running smoothly and adapting to your research needs. From startup essentials to long-term maintenance and chip-specific upgrades — find everything you need to power consistent, high-quality experiments.
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VitroInsertX

The adaptable chip inlay for seamless integration with a wide range of microfluidic chips in our chip chamber. Compatible, customizable, ready to use.
  • Chip inlay for using different chip formats
  • Enables flexible chip integration
  • Additional insert for the VitroFlow platform
  • Allows switching between different chip types on the same device
  • Ideal when working with multiple chips within one project

 

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VITROKit RenewFlow P4

Pump element kit for stable flow control in your VitroFlow.Bio
  • Pump refresh elements
  • Maintains precise flow control
  • Replacement tubing set for the pump system
  • Ensures reliable and stable microfluidic flow
  • Recommended interval for kit: every 3 months

 

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VITROKit Renew M5

Membrane renewal set for bubble trap maintenance.
  • Replacement membranes for the bubble trap
  • Ensures stable bubble-free operation
  • Includes 5 bubble trap membranes
  • Supports reliable system operation by preventing bubble interference
  • Replacement depends on use case; recommended at least four times per year
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VITROKit 100

Routine refill kit for running your VitroFlow.Bio
  • For cell culture media
  • Refill kit for regular operation
  • Replacement set for routine use
  • Recommended replacement interval: every 3 months
  • Used for ongoing operation of the system

 

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VITROKit 100+

Starter kit for the initial setup of your VitroFlow.Bio
  • For cell culture media
  • Starter set for initial system setup
  • Includes 100 media vials and handling tool
  • Used for the first system setup
  • Enables preparation and supply of culture media to the system

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